Recombinant Human PRKCZ protein, His & T7-tagged

• Cat.No.: PRKCZ-6885H
• Product Overview: Recombinant Human PRKCZ aa. (Pro404~Ser591 (Accession # Q05513)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.4kDa.
• Protein length: Pro404~Ser591
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PRKCZ protein kinase C zeta [ Homo sapiens (human) ]
• Official Symbol: PRKCZ
• Synonyms: PKC2; PKC-ZETA; protein kinase C zeta type; nPKC-zeta
• Gene ID: 5590
• mRNA Refseq: NM_001033581.2
• Protein Refseq: NP_001028753.1
• UniProt ID: Q05513