Recombinant Full Length Human nudix hydrolase 21 Protein, His&Flag&StrepII tagged

• Cat.No.: NUDT21-079H
• Product Overview: Recombinant human NUDT21 protein (O43809) (Met 1-Asn 227) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag&His&Twin Strep
• Protein Length: 1-227aa
• Description: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function.
• Tag: N-Twin strep and C-His&Flag
• Molecular Mass: 32.9 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: NUDT21 nudix hydrolase 21 [ Homo sapiens (human) ]
• Official Symbol: NUDT21
• Synonyms: NUDT21; nudix (nucleoside diphosphate linked moiety X)-type motif 21; cleavage and polyadenylation specific factor 5, 25 kD subunit, cleavage and polyadenylation specific factor 5, 25 kDa, CPSF5; cleavage and polyadenylation specificity factor subunit 5; CFIM25; nudix motif 21; CPSF 25 kDa subunit; pre-mRNA cleavage factor Im (25kD); pre-mRNA cleavage factor Im, 25kD subunit; pre-mRNA cleavage factor Im 25 kDa subunit; nucleoside diphosphate-linked moiety X motif 21; cleavage and polyadenylation specific factor 5, 25 kDa; cleavage and polyadenylation specific factor 5, 25 kD subunit; cleavage and polyadenylation specificity factor 25 kDa subunit; CPSF5; DKFZp686H1588
• Gene ID: 11051
• mRNA Refseq: NM_007006
• Protein Refseq: NP_008937
• MIM: 604978
• UniProt ID: O43809