Recombinant Full Length Human GZMH Protein, C-His-tagged

• Cat.No.: GZMH-01HFL
• Product Overview: Recombinant Full Length Human GZMH Protein, fused to His-tag at C-terminus, was expressed in NS0.

Specification

• Species: Human
• Source: NS0
• Tag: His
• Protein Length: Glu19-Leu246
• Description: Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme H's functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type (3). Human Granzyme H shows the highest amino acid identity (71%) to mouse Granzyme C (4). Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1-18), a propeptide (residues 19-20) and a mature chain (residues 21-246) (5-7). The pro-enzyme is expressed and purified. After being activated by active cathepsin C, rhGranzyme H cleaves a thioester substrate, which was described previously (8).
• Conjugation/Label: Unconjugated
• Form: Supplied as a 0.2 μm filtered solution in HEPES, NaCl and Brij-35.
• Bio-activity: Measured by its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzyl ester (Suc-FLF-SBzl), in the presence of 5,5'-Dithio-bis (2-nitrobenzoic acid) (DTNB).
  The specific activity is >5,000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 27 kDa
  SDS-PAGE: Multiple bands between 38-40 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Applications: Enzyme Activity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
• N-terminal Sequence: Glu19
• References: 1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Sedelies, K.A. et al. (2004) J. Biol. Chem. 279:26581.
  4. Sattar, R. et al. (2003) Biochem. Biophys. Res. Comm. 308:726.
  5. Meier, M. et al. (1990) Biochemistry 29:4042.
  6. Haddad, P. et al. (1991) Int. Immunol. 3:57.
  7. Klein, J.L. et al. (1990) Tissue Antigens 35:220.
  8. Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.

Gene Information

• Gene Name: GZMH granzyme H [ Homo sapiens (human) ]
• Official Symbol: GZMH
• Synonyms: GZMH; granzyme H; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2; CGL2; CTSGL2; GrzH
• Gene ID: 2999
• mRNA Refseq: NM_033423.5
• Protein Refseq: NP_219491.1
• MIM: 116831
• UniProt ID: P20718