Recombinant Full Length Human C3 Protein, C-Flag-tagged

• Cat.No.: C3-365HFL
• Product Overview: Recombinant Full Length Human C3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 187 kDa
• AA Sequence: MGPTSGPSLLLLLLTHLPLALGSPM YSIITPNILRLESEETMVLEAHDAQ GDVPVTVTVHDFPGKKLVLS SEKTVLTPATNHMGNVTFTIPANRE FKSEKGRNKFVTVQATFGTQVVEKV VLVSLQSGYLFIQTDKTIYT PGSTVLYRIFTVNHKLLPVGRTVMV NIENPEGIPVKQDSLSSQNQLGVLP LSWDIPELVNMGQWKIRAYY ENSPQQVFSTEFEVKEYVLPSFEVI VEPTEKFYYIYNEKGLEVTITARFL YGKKVEGTAFVIFGIQDGEQ RISLPESLKRIPIEDGSGEVVLSRK VLLDGVQNLRAEDLVGKSLYVSATV ILHSGSDMVQAERSGIPIVT SPYQIHFTKTPKYFKPGMPFDLMVF VTNPDGSPAYRVPVAVQGEDTVQSL TQGDGVAKLSINTHPSQKPL SITVRTKKQELSEAEQATRTMQALP YSTVGNSNNYLHLSVLRTELRPGET LNVNFLLRMDRAHEAKIRYY TYLIMNKGRLLKAGRQVREPGQDLV VLPLSITTDFIPSFRLVAYYTLIGA SGQREVVADSVWVDVKDSCV GSLVVKSGQSEDRQPVPGQQMTLKI EGDHGARVVLVAVDKGVFVLNKKNK LTQSKIWDVVEKADIGCTPG SGKDYAGVFSDAGLTFTSSSGQQTA QRAELQCPQPAARRRRSVQLTEKRM DKVGKYPKELRKCCEDGMRE NPMRFSCQRRTRFISLGEACKKVFL DCCNYITELRRQHARASHLGLARSN LDEDIIAEENIVSRSEFPES WLWNVEDLKEPPKNGISTKLMNIFL KDSITTWEILAVSMSDKKGICVADP FEVTVMQDFFIDLRLPYSVV RNEQVEIRAVLYNYRQNQELKVRVE LLHNPAFCSLATTKRRHQQTVTIPP KSSLSVPYVIVPLKTGLQEV EVKAAVYHHFISDGVRKSLKVVPEG IRMNKTVAVRTLDPERLGREGVQKE DIPPADLSDQVPDTESETRI LLQGTPVAQMTEDAVDAERLKHLIV TPSGCGEQNMIGMTPTVIAVHYLDE TEQWEKFGLEKRQGALELIK KGYTQQLAFRQPSSAFAAFVKRAPS TWLTAYVVKVFSLAVNLIAIDSQVL CGAVKWLILEKQKPDGVFQE DAPVIHQEMIGGLRNNNEKDMALTA FVLISLQEAKDICEEQVNSLPGSIT KAGDFLEANYMNLQRSYTVA IAGYALAQMGRLKGPLLNKFLTTAK DKNRWEDPGKQLYNVEATSYALLAL LQLKDFDFVPPVVRWLNEQR YYGGGYGSTQATFMVFQALAQYQKD APDHQELNLDVSLQLPSRSSKITHR IHWESASLLRSEETKENEGF TVTAEGKGQGTLSVVTMYHAKAKDQ LTCNKFDLKVTIKPAPETEKRPQDA KNTMILEICTRYRGDQDATM SILDISMMTGFAPDTDDLKQLANGV DRYISKYELDKAFSDRNTLIIYLDK VSHSEDDCLAFKVHQYFNVE LIQPGAVKVYAYYNLEESCTRFYHP EKEDGKLNKLCRDELCRCAEENCFI QKSDDKVTLEERLDKACEPG VDYVYKTRLVKVQLSNDFDEYIMAI EQTIKSGSDEVQVGQQRTFISPIKC REALKLEEKKHYLMWGLSSD
  FWGEKPNLSYIIGKDTWVEHWPEED ECQDEENQKQCQDLGAFTESMVVFG CPNTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus

Gene Information

• Gene Name: C3 complement C3 [ Homo sapiens (human) ]
• Official Symbol: C3
• Synonyms: ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
• Gene ID: 718
• mRNA Refseq: NM_000064.4
• Protein Refseq: NP_000055.2
• MIM: 120700
• UniProt ID: P01024

Reviews

02/23/2022

Expression efficiency is high. In our experiments, we found that the target protein can be synthesized quickly and effectively.

01/26/2022

Activity was not significantly different from the native protein, as expected.

03/23/2021

High purity, no obvious impurities.

Q&As

Have proteins been found to interact with C3 proteins?

Yes, a variety of proteins that interact with C3 proteins, including complement receptors, regulatory proteins, inflammatory factors, etc., have been discovered, and they are jointly involved in the regulation and functional execution of C3 proteins.

What is the relationship between C3 protein and infectious diseases?

C3 protein plays an important role in infectious diseases. It can play an anti-infection role by activating the complement system, directly killing pathogens or enhancing the ability of immune cells to destroy pathogens.

How is the research progress of C3 protein at present?

Research on C3 protein is going on. At present, it mainly focuses on the analysis of its function and regulatory mechanism, the identification of gene mutations associated with diseases, and the interaction with other complement proteins and immune cells, which provides a theoretical basis for the further development of diagnosis and treatment methods of related diseases.

What is the use of C3 protein in laboratory diagnostics?

C3 protein can be used as an aid in the diagnosis of certain immune-related diseases. Measuring the level of C3 protein in serum can reflect the activity of the immune system and the degree of inflammation.

What are the effects of post-translational modifications of C3 protein on its function?

Post-translational modifications of the C3 protein can affect its function. Phosphorylation, cleavage, methylation and other modifications can regulate the activity, stability and interaction ability of C3 proteins.

Have any drug treatments associated with C3 protein been identified?

Yes, some C3 protein-related drug treatments are under development, including drugs that regulate the degree of C3 protein activation, drugs that inhibit the overactivation of C3 protein, etc.