Recombinant Cynomolgus/Rhesus macaque CD27 protein, Mouse IgG2a Fc-tagged, low endotoxin

• Cat.No.: CD27-335C
• Product Overview: Recombinant Cynomolgus/Rhesus macaque CD27 protein (Ala20-Arg191)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).In the region Ala20-Arg191, the AA sequence of Cynomolgus and Rhesus macaque CD27 are homologus.

Specification

• Description: CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27/TNFRSF7 is found in most T-lymphocytes. CD27/TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
• Source: HEK293
• Species: Cynomolgus/Rhesus macaque
• Tag: mIgG2a
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 46.2 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 172
• Endotoxin: Less than 0.1 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD27
• Official Symbol: CD27
• Synonyms: TNFRSF7
• Gene ID: 712693
• mRNA Refseq: XM_005569906.2
• Protein Refseq: XP_005569963.1
• UniProt ID: G7PJM7