Recombinant Cynomolgus GFRAL protein, His-Avi-tagged

• Cat.No.: GFRAL-4633C
• Product Overview: Recombinant Cynomolgus GFRAL protein(XP_015304775.2)(Gln20-Glu351), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gln20-Glu351
• Tag: N-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in 20mM PB, 0.4M NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus GFRAL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM PB, 0.4M NaCl (pH 6.0).