Recombinant Cynomolgus GFRAL protein, His-Avi-tagged, Biotinylated

• Cat.No.: GFRAL-1717C
• Product Overview: Biotinylated Recombinant Cynomolgus GFRAL protein(XP_015304775.2)(Gln20-Glu351), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: N-His-Avi
• Tag: Non
• Protein Length: Gln20-Glu351
• Tag: N-His-Avi
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in 20mM PB, 400mM NaCl (pH 6.0).
• Bio-activity: Immobilized Cynomolgus GDF15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.