Recombinant Cattle NOS3 protein, His & T7-tagged

• Cat.No.: NOS3-7854C
• Product Overview: Recombinant Cattle NOS3 aa. (Ala522~Phe705 (Accession # P29473)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Cattle
• Source: E.coli
• Tag: His&T7
• Protein Length: Ala522~Phe705
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 24.1kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.8.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: NOS3 nitric oxide synthase 3 [ Bos taurus (cattle) ]
• Official Symbol: NOS3
• Synonyms: NOS3; nitric oxide synthase, endothelial; EC-NOS; NOS type III; NOSIII; cNOS; constitutive NOS; eNOS; endothelial NOS; endothelial nitric oxide synthase; nitric oxide synthase 3 (endothelial cell)
• Gene ID: 287024
• mRNA Refseq: NM_181037.3
• Protein Refseq: NP_851380.2
• UniProt ID: P29473