Recombinant Rat Nos3 protein, His-tagged

• Cat.No.: Nos3-7860R
• Product Overview: Recombinant Rat Nos3 aa. (Ala519~Phe702 (Accession # Q62600)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Ala519~Phe702
• Description: Enzyme that synthesizes Nitric oxide from L-arginine.
• Form: Freeze-dried powder
• Molecular Mass: 23kDa as determined by
  SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Nos3 nitric oxide synthase 3 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Nos3
• Synonyms: Nos3; nitric oxide synthase 3; eNos; nitric oxide synthase, endothelial; EC-NOS; NOS type III; NOSIII; cNOS; constitutive NOS; endothelial NOS; endothelial nitric oxide synthase 3; nitric oxide synthase 3, endothelial cell
• Gene ID: 24600
• mRNA Refseq: NM_021838.2
• Protein Refseq: NP_068610.1
• UniProt ID: Q62600