Recombinant Mouse Nos3 Protein, His&GST-tagged

• Cat.No.: Nos3-1836M
• Product Overview: Recombinant Mouse Nos3 Protein (Arg97-Lys485) with N-His&GST tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: GST&His
• Protein Length: Arg97-Lys485
• Description: Enables nitric-oxide synthase activity and scaffold protein binding activity. Involved in several processes, including heart development; negative regulation of muscle hyperplasia; and smooth muscle hyperplasia. Acts upstream of or within several processes, including lipopolysaccharide-mediated signaling pathway; nitric oxide biosynthetic process; and regulation of metal ion transport. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; cardiovascular system; genitourinary system; hemolymphoid system gland; and nervous system. Used to study aortic valve disease; essential hypertension; and persistent fetal circulation syndrome. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer's disease (multiple); artery disease (multiple); eye disease (multiple); reproductive organ cancer (multiple); and vasculitis (multiple). Orthologous to human NOS3 (nitric oxide synthase 3).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 73.7 kDa
  Accurate Molecular Mass: 74 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Nos3 nitric oxide synthase 3, endothelial cell [ Mus musculus (house mouse) ]
• Official Symbol: Nos3
• Synonyms: Nos3; nitric oxide synthase 3, endothelial cell; eNOS; Nos-3; ecNOS; 2310065A03Rik; nitric oxide synthase, endothelial; EC-NOS; NOS type III; NOSIII; cNOS; constitutive NOS; endothelial NOS; endothelial nitric oxide synthase; EC 1.14.13.39
• Gene ID: 18127
• mRNA Refseq: NM_008713
• Protein Refseq: NP_032739
• UniProt ID: P70313