Recombinant Bovine Pancreatic Trypsin Inhibitor

• Cat.No.: PTI-50B
• Product Overview: Recombinant Bovine Aprotinin produced inE.Coliis approximately 6,512 Dalton highly purified and designed specifically for cell culture applications, protein purification, diagnostic testing and pharmaceuticals. It is produced without the use of animal- or human-derived materials and is therefore free from risk of contaminating human or animal-derived viruses or prions.

Specification

• Cat. No.: PTI-50B
• Description: Aprotinin, also known as bovine pancreatic trypsin inhibitor, BPTI (Trasylol, Bayer) is a protein, that is used as medication administered by injection to reduce bleeding during complex surgery, such as heart and liver surgery. Its main effect is the slowing down of fibrinolysis, the process that leads to the breakdown of blood clots. The aim in its use is to decrease the need for blood transfusions during surgery, as well as end-organ damage due to hypotension (low blood pressure) as a result of marked blood loss.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered lyophilized powder.
• Purity: Greater than 90.0%, determind by Gel Electrophoresis.
• Formulation: The protein was lyophilized without any additives.
• Solubility: It is recommended to reconstitute the Aprotinin in sterile 18MO-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: 4.6 TIU/mg.
• Unit Defenition: One Trypsin Inhibitory Unit (TIU) is equal to the amount of inhibitor with the ability to inhibit two trypsin units by 50% where one trypsin unit will hydrolyze 1.0 mole of N-alpha-benzoyl-DL-Arginine-p-Nitroanilide (BAPNA) per minute at pH 7.8 at 25°C.
• Storage: Aprotinin although stable at room temperature for 3 weeks, should be stored at 4°C.
• Tag: Non

Gene Information

• Gene Name: PTI pancreatic trypsin inhibitor [ Bos taurus ]
• Synonyms: pancreatic trypsin inhibitor; PTI; BPI; BPTI; MGC148815; aprotinin; basic protease inhibitor
• Gene ID: 404172
• mRNA Refseq: NM_001001554
• Protein Refseq: NP_001001554
• UniProt ID: P00974