Recombinant Bovine NOS3 Protein

• Cat.No.: NOS3-003B
• Product Overview: Recombinant full length bovine NOS3 Protein (2-1205) without tag was expressed in E. coli.

Specification

• Description: Produces nitric oxide (NO).
• Source: E. coli
• Species: Bovine
• Molecular Mass: 134 kDa
• Protein length: 2-1205
• Purity: Partially pure
• Unit Definition: One unit of enzyme produces 1 nmol of nitric oxide per minute at 37 centigrade in 50 mM HEPES, pH 7.8, with 5 μM oxyhemoglobin, 1 mM calcium chloride, 20 μg/mL calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.
• Applications: Enzyme assay
• Stability: ≥ 2 years
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM HEPES, pH 7.8, with 0.5 mM DTT, 0.5 mM EDTA, 300 mM sodium chloride, 2 mM calcium chloride, 10 μM tetrahydro-L-biopterin (hydrochloride), and 20% glycerol

Gene Information

• Gene Name: NOS3 nitric oxide synthase 3 [ Bos taurus (cattle) ]
• Official Symbol: NOS3
• Synonyms: NOS3; nitric oxide synthase 3; nitric oxide synthase, endothelial; EC-NOS; NOS type III; NOSIII; cNOS; constitutive NOS; eNOS; endothelial NOS; endothelial nitric oxide synthase; nitric oxide synthase 3 (endothelial cell); EC 1.14.13.39
• Gene ID: 287024
• mRNA Refseq: NM_181037
• Protein Refseq: NP_851380
• UniProt ID: A0A3Q1LSD1