Rabbit Anti-Human Dickkopf 1 Homolog Monoclonal Antibody

• Cat.No.: CAB11524RH
• Product Overview: Rabbit Monoclonal Antibody to Human Dickkopf 1 Homolog

Specification

• Species: Human
• Source: Rabbit
• Tag: Non
• Antigen Description: DKK1 ( dickkopf related protein 1 ) is a head inducer secreted from the vertebrate head organizer and belongs to the DKK protein family including four members. DKK1 is synthesized initially as a precursor protein with an N-terminal signal peptide and two cysteine-rich domains. DKK proteins have distinct patterns of expression in adult and embryonic tissues and have effects on Wnt signaling implicated in diverse processes. Dkk1 is a specific and high-affinity ligand for LRP6 ( LDL-receptor-related protein 6 ) acting as a Wnt co-receptor. Dkk1 blocks LRP6-mediated Wnt/beta-catenin signaling by antagonistically inhibiting the formation of Wnt-Fz-LRP6 complex which triggers the Wnt signal pathway. It is revealed that Wnt signaling in osteoblasts is necessary to promote BMP-2-mediated differentiation of pre-osteoblast cells, and therefore, DKK1 may play a key role in regulating pre-osteoblast differentiation and the development of multiple myeloma-associated osteolytic bone lesions.
• Specificity: Human Dkk1 / Dickkopf-1. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
• Immunogen: Recombinant human Dkk1 protein
• Isotype: Rabbit IgG
• Clone: A005
• Applications: WB; ELISA
• Dilution: Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human Dkk1 in WB. Using a DAB detection system, the detection limit for Dkk1 is approximately 1 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommendedELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human Dkk1. The detection limit for human Dkk1 is approximately 0.00975 ng/well
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human dickkopf related protein 1.
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
• Official Symbol: DKK1
• Synonyms: DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1
• Gene ID: 22943
• mRNA Refseq: NM_012242
• Protein Refseq: NP_036374
• MIM: 605189
• UniProt ID: O94907
• Chromosome Location: 10q11.2
• Pathway: Direct p53 effectors, organism-specific biosystem; Presenilin action in Notch and Wnt signaling, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem; Wnt Signaling Pathway NetPath, organism-specific biosystem; Wnt signaling network, organism-specific biosystem; Wnt signaling pathway, organism-specific biosystem
• Function: growth factor activity; low-density lipoprotein particle receptor binding; protein binding; receptor antagonist activity; signal transducer activity