Myc CoIP Agarose

• Cat.No.: Myc-01A
• Product Overview: Immunoprecipitation of Myc-tagged proteins and their interacting factors with anti-Myc Nanobody conjugated to beads.
  • Fast, reliable & efficient one-stepImmunoprecipitation
  • Elution with peptide
  • No heavy & light antibody chains
  • Stable under harsh washing conditions
  • Suitable for downstream mass spec analysis

Specification

• Specificity/Target: Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
• Binding capacity: 17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads)
• Elution buffer: 2x Myc peptide.
  SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Affinity (KD): 1x Myc-tag:Dissociation constant KD of 500 nM
  2x Myc-tag:Dissociation constant KD of 0.5 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP
• Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!