Mouse anti-HBe Monoclonal Antibody(coating)

• Cat.No.: CAB045

Specification

• Cat. No.: CAB045
• Antigen information: Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA
• Specificity: HBe-Ag
• Clone: LT03
• Host Animal: Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
• Immunogen: hepatitis virus granule
• Source: Ascites
• Format: Purified, Liquid
• Purification: >90% pure (SDS-PAGE). Protein A chromatography
• Concentration: 4mg/ml (OD280nm, E^0.1%= 1.4)
• Affinity Constant: 1X10¹¹L/Mol
• Buffer: PBS, pH 7.4
• Preservative: 0.1% NaN3
• Applications: Detection for HBe antigen. Suitable for use in ELISA, Western-Blotting and tissue immunity, Suggested pair for ELISA immunoassay: Capture DetectionCAB045 CAB046
• Storage: Short term at 2-8°C,Long term at -20°C
• Tag: Non