Active Recombinant Full Length Human PPM1A, His-tagged

• Cat.No.: PPM1A-66H
• Product Overview: Recombinant human PP2Cα was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques,46.6 kDa (418 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-382 a.a.
• Description: Protein phosphatase 2C(PP2Cα) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2Cα (amino acids 1-382) was cloned into an E. coli expression vector.
• Sequences of amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW
• Formulation: Liquid. In 10 mM Tris-HCl, pH7.5, 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20%glycerol.
• Molecular Weight: 46.6 kDa (418 aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method)
• Specific Activity: >8,000 units/mg of PP2C alpha
• Unit Difinition: One unit will hydrolyze 1nanomole of p-nitrophenyl phosphatate(pNPP) per minute at pH 7.4 at 37℃ using 10mM of substrate
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publications:
  Protein phosphatase magnesium-dependent 1A induces inflammation in rheumatoid arthritis (2020)
  Role of Protein Phosphatase Magnesium-Dependent 1A and Anti–Protein Phosphatase Magnesium-Dependent 1A Autoantibodies in Ankylosing Spondylitis (2014)

Gene Information

• Gene Name: PPM1A protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform [ Homo sapiens ]
• Synonyms: Protein phosphatase 1A; EC 3.1.3.16, Protein phosphatase 2C isoform alpha; PP2C-alpha; IA; PPM1A; PP2CA; MGC9201; PPP2CA; PP2Ac; RP-C; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isoform; PP2A-alpha; replication protein C; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; FLJ42306; PP2C-ALPHA; PP2C-alpha; protein ph; protein phosphatase1A (formerly 2C), magnesium-dependent, alpha isoform
• Gene ID: 5494
• mRNA Refseq: NM_021003
• Protein Refseq: NP_066283
• MIM: 606108
• UniProt ID: P35813
• Chromosome Location: 14q23.1
• Function: hydrolase activity; magnesium ion binding; manganese ion binding; protein C-terminus binding; protein serine/threonine phosphatase activity; signal transducer activity