Recombinant Human PPM1A Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PPM1A-4860H
• Product Overview: PPM1A MS Standard C13 and N15-labeled recombinant protein (NP_066283) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
• Molecular Mass: 42.4 kDa
• AA Sequence: MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PPM1A protein phosphatase, Mg2+/Mn2+ dependent 1A [ Homo sapiens (human) ]
• Official Symbol: PPM1A
• Synonyms: PPM1A; protein phosphatase, Mg2+/Mn2+ dependent, 1A; protein phosphatase 1A (formerly 2C), magnesium dependent, alpha isoform; protein phosphatase 1A; MGC9201; phosphatase 2C alpha; PP2CA; PP2Calpha; protein phosphatase 2C; alpha isoform; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform; PP2C-ALPHA; FLJ42306
• Gene ID: 5494
• mRNA Refseq: NM_021003
• Protein Refseq: NP_066283
• MIM: 606108
• UniProt ID: P35813