Recombinant Full Length Mouse Ppm1a Protein, Myc/DDK-tagged

• Cat.No.: Ppm1a-5045M
• Product Overview: Purified recombinant protein of mouse full-length protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
• Molecular Mass: 42.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ppm1a protein phosphatase 1A, magnesium dependent, alpha isoform [ Mus musculus (house mouse) ]
• Official Symbol: Ppm1a
• Synonyms: PPM1A; protein phosphatase 1A, magnesium dependent, alpha isoform; protein phosphatase 1A; Mpp alpha; PP2C-alpha; protein phosphatase IA; protein phosphatase 2C isoform alpha; MMPa-2; MPPa-1; AI427932; AU017636; 2310003C21Rik; 2900017D14Rik
• Gene ID: 19042
• mRNA Refseq: NM_008910
• Protein Refseq: NP_032936
• UniProt ID: P49443