Cathepsin L Inhibitor Screening Kit (Fluorometric)

• Cat.No.: Kit-2351
• Product Overview: Cathepsin L (CTSL, EC 3.4.22.15) is a lysosomal cysteine protease that is implicated in protein degradation, arthritis, apoptosis, and cancer. Cathepsin L Inhibitor Screening Kit utilizes the ability of active Cathepsin L to cleave the synthetic AFC-based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin L inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin L.

Specification

• Tag: Non
• Applications: Screen/study/characterize potential inhibitors of Cathepsin L
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: CTSL Assay Buffer; CTSL Reagent; DTT; Cathepsin L (human, 20 mU); CTSL Substrate, Ac-FR-AFC (10 mM); CTSL Inhibitor (FF-FMK, 1 mM)
• Detection method: Fluorescence (Ex/Em 400/505 nm)
• Features & Benefits: Convenient & Rapid;
  High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin L;
  Cathepsin L inhibitor, FF-FMK is included in the kit