Active Recombinant Human CTSL Protein, His-tagged

• Cat.No.: CTSL1-405H
• Product Overview: Recombinant human CTSL (Glu113-Val333 & Ala114-Val333) protein with His tag at C-terminal was expressed in mouse myeloma cell line.

Specification

• Description: The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. The specific activity is >25,000 pmol/min/μg.
• Molecular Mass: 26 kDa
• N-terminal Sequence Analysis: Glu113 & Ala114
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
• Protein length: 113-333;114-333 a.a.

Gene Information

• Gene Name: CTSL cathepsin L [ Homo sapiens (human) ]
• Official Symbol: CTSL
• Synonyms: CTSL; cathepsin L; MEP; CATL; CTSL1; cathepsin L1; major excreted protein; EC 3.4.22.15
• Gene ID: 1514
• mRNA Refseq: NM_145918
• Protein Refseq: NP_666023
• MIM: 116880
• UniProt ID: P07711