Active Recombinant Zebrafish tek Protein, Fc Chimera

• Cat.No.: tek-1020Z
• Product Overview: Recombinant Zebrafish tek (Accession # O73791), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Zebrafish
• Tag: Fc
• Predicted N Terminal: Val22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized rzfTie-2/Fc Chimera at 4 µg/mL (100 µL/well) can binds rhAngiopoietin-2 with a linear range of 2 - 100 ng/mL.
• Molecular Mass: Recombinant Zebrafish tek, Fc Chimera has a calculated MW of 104.4 kDa (monomer). In SDS-PAGE migrates as 122 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: tek TEK tyrosine kinase, endothelial [ Danio rerio (zebrafish) ]
• Official Symbol: tek
• Synonyms: tie2; tie-2; tyrosine-protein kinase receptor Tie-2; tyrosine-protein kinase receptor Tie-2; endothelium-specific receptor tyrosine kinase 2; tyrosine kinase with Ig and EGF homology domains-2
• Gene ID: 30747
• mRNA Refseq: NM_131461.1
• Protein Refseq: NP_571536.1
• UniProt ID: O73791
• Chromosome Location: chromosome: 5