Active Recombinant Thioredoxin, His-tagged

• Cat.No.: TXN-4662H
• Product Overview: Thioredoxin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids (1-105 a.a.) and having a molecular mass of 13.9 kDa. TXN protein is fused to a 20 amino acid His-Tag at N-terminus and purified by standard chromatography.

Specification

• Species: Human
• Source: Human
• Tag: His
• Protein Length: 1-105 a.a.
• Description: Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
• Form: TXN1 solution containing 1x PBS pH 7.4.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Bio-activity: Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
• Physical Appearance: Sterile filtered colorless solution.
• Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV.
• Storage: Thioredoxin human although stable at 4ºC for 1 week, should be stored desiccated below -18ºC. Please prevent freeze thaw cycles.
• Pathways: Nucleotide metabolism

Gene Information

• Gene Name: TXN thioredoxin [ Homo sapiens ]
• Official Symbol: TXN
• Synonyms: TXN; MGC61975; TRX; ADF; DKFZp686B1993; MGC61975; OTTHUMP00000021892; SASP; TRDX; TRX1; Trx; thioredoxin; ATL-derived factor; Surface-associated sulphydryl protein
• Gene ID: 7295
• mRNA Refseq: NM_003329
• Protein Refseq: NP_003320
• MIM: 187700
• UniProt ID: P10599
• Chromosome Location: 9q31
• Function: protein binding