Active Recombinant Streptococcus pyogenes IdeS protein, His&GST-tagged

• Cat.No.: IdeS-2216S
• Product Overview: Recombinant Streptococcus pyogenes IdeS protein(F8V4V0-1)(Asp30-Asn341), fused with His&GST tag, was expressed in E. coli.

Specification

• Species: Streptococcus pyogenes
• Source: E.coli
• Tag: His&GST
• ProteinLength: Asp30-Asn341
• Form: Supplied as sterile PBS, 50 % glycerol, pH 7.4.
• Bio-activity: One unit digests ≥ 95% of 1µg human IgG when incubated in PBS buffer, pH 7.4 at 37°C for 30min. The specific activity is 20,000 unit/mg.
• Molecular Mass: The recombinant streptococcus pyogenes IdeS consists of 549 amino acids and predicts a molecular mass of 63.1 kDa.
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.