Active Recombinant Rhesus IL2RB protein(Met1-Asp239), hFc-tagged

• Cat.No.: IL2RB-454R
• Product Overview: Recombinant rhesus IL2RB (NP_001244989.1) (Met1-Asp239) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rhesus
• Tag: Fc
• Protein length: Met1-Asp239
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit IL-15-dependent prolifeRation of MO7e human megakaryocytic leukemic cells in the presence of 4 ng/mL of recombinant human IL-15. The ED50 for this effect is typically 0.4-2.4 μg/mL.
• Molecular Mass: The recombinant rhesus IL2RB consists of 451 amino acids and predicts a molecular mass of 51.4 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.