Active Recombinant Rat IL2RB protein, His&Twin-Strep-tagged

• Cat.No.: IL2RB-951R
• Product Overview: Recombinant Rat IL2RB protein(Ala 27 - Glu 239 (IL-2RB) & Glu 22 - Gln 235 (IL-2RA) & Ser 25 - Ala 262 (IL-2RG)), fused with His and Twin-Strep tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His&Twin Strep
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human IL-2, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag with a linear range of 39-156 ng/mL.
• Molecular Mass: Molecular CharacterizationRat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 30.2 kDa (IL-2RB) and 59.3 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a his tag at the C-terminus and subunit IL-2RA&IL-2RG is fused with a Twin-Strep Tag at the C-terminus. The reducing (R) protein migrates as 45 kDa and 110 kDa respectively due to glycosylation.
• Proteinlength: Ala27-Glu239(IL-2RB)&Glu22-Gln235(IL-2RA)&Ser25-Ala262(IL-2RG)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 27-239;22-235;25-262 a.a.

Gene Information

• Gene Name: Il2rb interleukin 2 receptor, beta [ Rattus norvegicus ]
• Official Symbol: IL2RB
• Synonyms: IL2RB; interleukin 2 receptor, beta; interleukin-2 receptor subunit beta; IL-2RB; P70-75; IL-2R subunit beta; IL-2 receptor subunit beta; interleukin 2 receptor, beta chain; high affinity IL-2 receptor subunit beta; IL2RBC
• Gene ID: 25746
• mRNA Refseq: NM_013195
• Protein Refseq: NP_037327