Active Recombinant Rat TIMP1 Protein

• Cat.No.: TIMP1-1072R
• Product Overview: Recombinant rat TIMP1 (P01860) (Met1-Ala217) was expressed and purified.

Specification

• Species: Rat
• Source: HEK293
• Tag: Non
• Protein Length: 1-217 a.a.
• Predicted N Terminal: Cys 24
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.0, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate MCA-PLGLDPA-AR-NH2. The IC50 value is approximately 4 nM.
• Molecular Mass: The recombinant rat TIMP1 consists of 194 amino acids and has a predicted molecular mass of 21.5 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 28 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >97 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Timp1 TIMP metallopeptidase inhibitor 1 [ Rattus norvegicus ]
• Official Symbol: TIMP1
• Synonyms: TIMP1; TIMP metallopeptidase inhibitor 1; metalloproteinase inhibitor 1; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinases 1; Timp; TIMP-1
• Gene ID: 116510
• mRNA Refseq: NM_053819
• Protein Refseq: NP_446271
• Pathway: Hemostasis, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; Platelet degranulation, organism-specific biosystem; Response to elevated platele
• Function: enzyme inhibitor activity; metal ion binding; metalloendopeptidase inhibitor activity; metalloendopeptidase inhibitor activity