Recombinant Human TIMP1, His-tagged

• Cat.No.: TIMP1-77H
• Product Overview: Recombinant human TIPM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: TIMP1, also known as TIMP metalloproteinase inhibitor 1, is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function.
• Form: Liquid. In phosphate buffered saline (pH7.4).
• Molecular Mass: 21.5kDa (190aa) 18-28KDa (SDS-PAGE under reducing conditions.)
• AA Sequence: CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIAHHHHHH
• Endotoxin: < 1.0 eu per 1 microgram of protein (determined by lal
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.2mg/ml (determined by Bradford)

Gene Information

• Gene Name: TIMP1 TIMP metallopeptidase inhibitor 1 [ Homo sapiens ]
• Official Symbol: TIMP1
• Synonyms: TIMP1; TIMP metallopeptidase inhibitor 1; CLGI, TIMP, tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); metalloproteinase inhibitor 1; EPO; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1; EPA; HCI; CLGI; TIMP; FLJ90373
• Gene ID: 7076
• mRNA Refseq: NM_003254
• Protein Refseq: NP_003245
• MIM: 305370
• UniProt ID: P01033
• Chromosome Location: Xp11.3-p11.23
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Hemostasis, organism-specific biosystem; IL6-mediated signaling events, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem
• Function: enzyme inhibitor activity; metal ion binding; metalloendopeptidase inhibitor activity; metalloendopeptidase inhibitor activity; protein binding