Active Recombinant Rat renin Protein, Tag Free

• Cat.No.: Ren-47R
• Product Overview: Recombinant rat pro-renin was expressed in HEK cells. Purified enzyme was converted to the active renin by tryptic activation followed by removal of trypsin. The activity of enzyme can be measured in FRET-based assays

Specification

• Description: Enables endopeptidase activity and insulin-like growth factor receptor binding activity. Involved in several processes, including peptide metabolic process; response to lipopolysaccharide; and response to purine-containing compound. Located in cytoplasm and extracellular space. Used to study hypertension. Biomarker of primary biliary cholangitis. Human ortholog(s) of this gene implicated in essential hypertension; familial juvenile hyperuricemic nephropathy; hand, foot and mouth disease; hypertension; and portal hypertension. Orthologous to human REN (renin).
• Source: HEK293
• Species: Rat
• Tag: Non
• Protein length: 65-402aa
• Form: Solution
• Molecular Mass: 38.5 kDa
• Purity: ≥90% by SDS-PAGE
• Applications: ELISA; FRET; Immunoprecipitation (IP); Protease activity
• Usage: Research use
• Unit Definition: One unit of protease hydrolyzes 1 picomole of FRET peptide (ArgGlu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Il2-His-Thr-Lys(Dabcyl)-Arg) per minute
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 0.5 mg/mL
• Storage Buffer: 50 mM Tris-HCl, pH 8.0
• References: 1. He, FJ. and GA. MacGregor, J. Renin Angiotensin Aldosterone Syst. 4, 11 (2003).
  2. Hollenberg, NK. J. Am. Soc. Nephrol. 10, S239 (1999).
  3. Paschalidou, K. et al., Biochem. J. 382, 1031 (2004).

Gene Information

• Gene Name: Ren renin [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Ren
• Synonyms: Ren; renin; Ren1; RENAA; RATRENAA; renin; angiotensinogenase; renin 1 structural; EC 3.4.23.15
• Gene ID: 24715
• mRNA Refseq: NM_012642
• Protein Refseq: NP_036774
• UniProt ID: P08424