Active Recombinant Rat IL21 protein(Met1-Ser146), His-tagged

• Cat.No.: IL21-3378R
• Product Overview: Recombinant Rat IL21 (NP_001102413.1) (Met1-Ser146) was expressed in Insect Cells with a polyhistide tag at the C-terminus.

Specification

• Species: Rat
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Ser146
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 2-8 ng/ml.
• Molecular Mass: The recombinant rat IL21 consists of 140 amino acids and predicts a molecular mass of 16.64 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il21 interleukin 21 [ Rattus norvegicus ]
• Official Symbol: IL21
• Synonyms: IL21; interleukin 21; interleukin-21; IL-21
• Gene ID: 365769
• mRNA Refseq: NM_001108943
• Protein Refseq: NP_001102413