Active Recombinant Mouse Il21 Protein

• Cat.No.: Il21-3511M
• Product Overview: Purified recombinant protein of Mouse interleukin 21 (Il21) without tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: Pro25-Ser146
• Description: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG1 and IgG3 in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
• Bio-activity: The ED50 is 15 - 60 ng/mL, corresponding to a specific activity of 1.7 - 6.7 × 10^4 units/mg.
• Molecular Mass: 14.2 kDa
• Endotoxin: Less than 0.01 ng/μg protein as determined by the LAL method
• Purity: > 98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 μg/mL in specified buffer
• Storage Buffer: 1 x PBS

Gene Information

• Gene Name: Il21 interleukin 21 [ Mus musculus (house mouse) ]
• Official Symbol: Il21
• Synonyms: IL21; interleukin 21; interleukin-21; IL-21
• Gene ID: 60505
• mRNA Refseq: NM_021782
• Protein Refseq: NP_068554
• UniProt ID: Q9ES17