Active Recombinant Rat Ephb3 Protein, His-tagged

• Cat.No.: Ephb3-1160R
• Product Overview: Recombinant rat Ephb3 (NP_001099338.1) (Met1-Thr537) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 1-537 a.a.
• Predicted N Terminal: Leu 30
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rat EPHB3-His at 10 μg/ml (100 μl /well) can bind rat EFNB1-Fc, The EC50 of rat EFNB1-Fc is 2-6ng/ml.
• Molecular Mass: The recombinant rat Ephb3 consists 519 amino acids and predicts a molecular mass of 57 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Ephb3 Eph receptor B3 [ Rattus norvegicus ]
• Official Symbol: Ephb3
• Synonyms: EPHB3; Eph receptor B3; ephrin type-B receptor 3; EPH-like tyrosine kinase-2
• Gene ID: 287989
• mRNA Refseq: NM_001105868
• Protein Refseq: NP_001099338
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: ATP binding; axon guidance receptor activity; axon guidance receptor activity; ephrin receptor activity; ephrin receptor activity; nucleotide binding; protein tyrosine kinase activity; receptor activity