Active Recombinant Mouse EphB3 Protein (Leu30-Thr537), C-Fc and 6×His tagged

• Cat.No.: Ephb3-29M
• Product Overview: Recombinant Mouse EphB3 protein (Leu30-Thr537) with Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Specification

• Description: Enables axon guidance receptor activity. Involved in several processes, including corpus callosum development; neuron projection development; and tube morphogenesis. Acts upstream of or within central nervous system projection neuron axonogenesis and retinal ganglion cell axon guidance. Predicted to be located in cell projection and plasma membrane. Predicted to be integral component of membrane. Predicted to be part of receptor complex. Predicted to be active in neuron projection. Predicted to be integral component of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; embryo mesenchyme; heart; and sensory organ. Orthologous to human EPHB3 (EPH receptor B3).
• Source: Mouse myeloma cell line
• Species: Mouse
• Tag: His&Fc
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse EphB3 Fc Chimera at 2 μg/mL (100 μL/well) can bind recombinant mouse Ephrin-B1 Fc Chimera with a linear range of 0.078-1.25 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 82.8 kDa (monomer)
  SDS-PAGE: 100 kDa, reducing conditions
• Protein length: Leu30-Thr537
• N-terminal Sequence Analysis: Leu30
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Ephb3 Eph receptor B3 [ Mus musculus (house mouse) ]
• Official Symbol: Ephb3
• Synonyms: EPHB3; Eph receptor B3; ephrin type-B receptor 3; mDK-5; developmental kinase 5; tyrosine-protein kinase receptor SEK-4; Etk2; HEK2; MDK5; Sek4; Cek10; Tyro6; AW456895
• Gene ID: 13845
• mRNA Refseq: NM_010143
• Protein Refseq: NP_034273
• UniProt ID: P54754