Active Recombinant Rat Eph Receptor B1, Fc-tagged

• Cat.No.: Ephb1-468R
• Product Overview: Recombinant Rat Ephb1protein was expressed in Murine myeloma cell line with the Fc part of human IgG1.Met18-Gln538.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 18-538 a.a.
• Description: Ephb1, also known asElk, Cek6, Net, and Hek6, is a member of the Eph receptor family which binds membersof the ephrin ligand family. There are two classes of receptors, designated Aand B. Both the A and B class receptors have an extracellular region consistingof a globular domain, a cysteinerich domain, and two fibronectin type III domains.This is followed by the transmembrane region and the cytoplasmic region.
• Form: Lyophilized from a 0.2μm filtered solution in Tris.
• N-terminalSequence: Met18
• Molecular Weight: 85 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rrEphb1/Fc Chimera at2 μg/mL (100 μL/well) can bind rmEphrinb1/Fc Chimera with a linear range of 0.31-20ng/mL. Optimal dilutions should be determined by each laboratory for each application.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Ephb1 Eph receptorB1 [ Rattus norvegicus ]
• Official Symbol: Ephb1
• Synonyms: Ephb1; Ephreceptor B1; Erk; elk; Ephb2; ephrin type-B receptor 1; ELK-related protein tyrosinekinase; tyrosine-protein kinase receptor EPH-2; Eph receptor B2 (ELK-related proteintyrosine kinase); EC 2.7.10.1; Epth2
• Gene ID: 24338
• mRNA Refseq: NM_001104528
• Protein Refseq: NP_001097998
• UniProt ID: P09759
• Chromosome Location: 8q32
• Pathway: Axon guidance
• Function: ATP binding; axon guidancereceptor activity; ephrin receptor activity; nucleotide binding; protein tyrosinekinase activity; receptor activity; transmembrane-ephrin receptor activity; transferaseactivity