Active Recombinant Mouse EPHB1, GST-tagged

• Cat.No.: EPHB1-1401M
• Product Overview: Recombinant mouse EPHB1 (591-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Species: Mouse
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 591 aa-end
• Description: EPHB1 is a member of the Eph family of receptor tyrosine kinases. Ligand-activated EPHB1 forms a signaling complex with Nck, paxillin, and focal adhesion kinase and induces tyrosine phosphorylation of paxillin in a c-Src-dependent manner to promote cell migration. In addition, activated EPHB1 recruits the adaptor proteins Grb2 and p52Shc and promotes p52Shc and c-Src tyrosine phosphorylation as well as MAPK/extracellular signal-regulated kinase (ERK) activation. Expression of dominant-negative c-Src significantly reduced EPHB1-dependent ERK1/2 activation and chemotaxis.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 143 nmol/min/mg
• Molecular Mass: ~62kDa
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: Ephb1 Eph receptor B1 [ Mus musculus ]
• Official Symbol: EPHB1
• Synonyms: EPHB1; Eph receptor B1; Elk
• Gene ID: 13843