Active Recombinant Human EPHB1 protein, His&GST-tagged

• Cat.No.: EPHB1-8550H
• Product Overview: Recombinant Human EPHB1 protein(AAI11745.1)(Arg565-Ala984), fused with His&GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Arg565-Ala984
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 3mM DTT, pH 8.0, 10% glyPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be 140 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
• Molecular Mass: The recombinant human EPHB1 /GST chimera consists of 657 amino acids and has a calculated molecular mass of 75.3 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHB1 EPH receptor B1 [ Homo sapiens ]
• Official Symbol: EPHB1
• Synonyms: EPHB1; EPH receptor B1; EphB1 , EPHT2; ephrin type-B receptor 1; Hek6; EK6; EPH-like kinase 6; eph tyrosine kinase 2; soluble EPHB1 variant 1; tyrosine-protein kinase receptor EPH-2; neuronally-expressed EPH-related tyrosine kinase; ELK; NET; EPHT2; FLJ37986
• Gene ID: 2047
• mRNA Refseq: NM_004441
• Protein Refseq: NP_004432
• MIM: 600600
• UniProt ID: P54762