Active Recombinant Rat Egf protein

• Cat.No.: Egf-550R
• Product Overview: Recombinant Rat Egf protein(NP_036974.1)(Asn974-Arg1026) was expressed in E. coli.

Specification

• Source: E. coli
• Species: Rat
• Tag: Non
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts.The ED50 for this effect is typically 0.05-0.3ng/mL.
• Molecular Mass: The recombinant rat EGF consists of 54 amino acids and has a predicted molecular mass of 6.3 kDa. The apparent molecular mass of the recombinant protein is approximately 6-9 kDa in SDS-PAGE under reducing conditions.
• Protein length: Asn974-Arg1026
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Egf epidermal growth factor [ Rattus norvegicus ]
• Official Symbol: Egf
• Synonyms: EGF; epidermal growth factor; pro-epidermal growth factor
• Gene ID: 25313
• mRNA Refseq: NM_012842
• Protein Refseq: NP_036974