Recombinant Mouse Egf protein, His & GST-tagged

• Cat.No.: Egf-2814M
• Product Overview: Recombinant Mouse Egf aa. (Asn977~Arg1029) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis.
• Source: E. coli
• Species: Mouse
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Asn977~Arg1029
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.3.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Egf epidermal growth factor [ Mus musculus (house mouse) ]
• Official Symbol: Egf
• Synonyms: AI790464; Pro-epidermal growth factor precursor (EGF); pro-epidermal growth factor
• Gene ID: 13645
• mRNA Refseq: NM_001310737.1
• Protein Refseq: NP_001297666.1
• UniProt ID: A0A0G2JF92