Active Recombinant Mouse Tnfsf12 Protein, Met & His-tagged

• Cat.No.: Tnfsf12-983M
• Product Overview: Recombinant Mouse Tnfsf12 (Accession # O54907.2) Arg105-His249, fused with an N-terminal Met and a 6-His tag, was produced in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Arg105-His249
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 0.03 - 0.3 µg/mL.Measured in a cytotoxicity assay using HT-29 human colon adenocarcinoma cells Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733; Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548; Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.The ED50 for this effect is typically 0.1 - 0.4 µg/mL.
• Molecular Mass: Recombinant Mouse Tnfsf12, Met & His-tagged has a calculated MW of 17 kDa.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Tnfsf12 tumor necrosis factor (ligand) superfamily, member 12 [ Mus musculus ]
• Official Symbol: Tnfsf12
• Synonyms: TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis; Dr3l; Apo3l; Dr3lg; Tweak
• Gene ID: 21944
• mRNA Refseq: NM_011614
• Protein Refseq: NP_035744