Active Recombinant Mouse Thpo protein, His-tagged

• Cat.No.: Thpo-97M
• Product Overview: Recombinant Mouse Thpo protein(NP_033405.1)(Met1-Thr356), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-356 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 1.6-6.4 ng/mL.
• Molecular Mass: The recombinant mouse THPO consists of 346 amino acids with the predicted molecular mass of 37 kDa. As a result of glycosylation, rmTHPO migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Thr356
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Thpo thrombopoietin [ Mus musculus ]
• Official Symbol: Thpo
• Synonyms: THPO; thrombopoietin; C-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand; Ml; Tpo; Mgdf; Tpo1; Tpo2; Tpo3; Tpo4; Mpllg
• Gene ID: 21832
• mRNA Refseq: NM_001173505
• Protein Refseq: NP_001166976