Active Recombinant Mouse Spint1 Protein, His-tagged

• Cat.No.: Spint1-706M
• Product Overview: Recombinant Mouse Spint1 (Accession # NP_058603) Glu30-Glu443, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Glu30
• Form: Lyophilized from a 0.2 µm filtered solution in Tris, NaCl and CaCl2.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The IC50 value is <1.5 nM, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Spint1, His-tagged has a calculated MW of 48 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
• Protein length: Glu30-Glu443
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Spint1 serine protease inhibitor, Kunitz type 1 [ Mus musculus ]
• Official Symbol: Spint1
• Synonyms: SPINT1; serine protease inhibitor, Kunitz type 1; kunitz-type protease inhibitor 1; hepatocyte growth factor activator inhibitor type 1; HAI-1
• Gene ID: 20732
• mRNA Refseq: NM_016907
• Protein Refseq: NP_058603