Active Recombinant Human SPINT1 Protein, His-tagged
Cat.No. : | SPINT1-540H |
Product Overview : | Recombinant Human SPINT1 (Accession # NP_003701) Pro37-Glu449, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived. |
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Source : | Mammalian cells |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Pro37 |
Form : | Lyophilized from a 0.2 µm filtered solution in HEPES, NaCl and CaCl2. |
Bio-activity : | Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The IC50 is <2 nM, as measured under the described conditions. |
Molecular Mass : | Recombinant Human SPINT1, His-tagged has a calculated MW of 47 kDa. In SDS-PAGE migrates as 58 kDa, reducing conditions. |
Protein length : | Pro37-Glu449 |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Gene Name | SPINT1 serine peptidase inhibitor, Kunitz type 1 [ Homo sapiens ] |
Official Symbol | SPINT1 |
Synonyms | SPINT1; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; kunitz-type protease inhibitor 1; HAI; MANSC2; HAI-1; hepatocyte growth factor activator inhibitor type 1; HAI1; |
Gene ID | 6692 |
mRNA Refseq | NM_001032367 |
Protein Refseq | NP_001027539 |
MIM | 605123 |
UniProt ID | O43278 |
Chromosome Location | 15q13.3 |
Not For Human Consumption!
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