Active Recombinant Human SPINT1 Protein, His-tagged

• Cat.No.: SPINT1-540H
• Product Overview: Recombinant Human SPINT1 (Accession # NP_003701) Pro37-Glu449, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Pro37-Glu449
• Predicted N Terminal: Pro37
• Form: Lyophilized from a 0.2 µm filtered solution in HEPES, NaCl and CaCl2.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The IC50 is <2 nM, as measured under the described conditions.
• Molecular Mass: Recombinant Human SPINT1, His-tagged has a calculated MW of 47 kDa. In SDS-PAGE migrates as 58 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SPINT1 serine peptidase inhibitor, Kunitz type 1 [ Homo sapiens ]
• Official Symbol: SPINT1
• Synonyms: SPINT1; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; kunitz-type protease inhibitor 1; HAI; MANSC2; HAI-1; hepatocyte growth factor activator inhibitor type 1; HAI1
• Gene ID: 6692
• mRNA Refseq: NM_001032367
• Protein Refseq: NP_001027539
• MIM: 605123
• UniProt ID: O43278
• Chromosome Location: 15q13.3