Active Recombinant Human SPINT1 Protein, His-tagged

Cat.No. : SPINT1-540H
Product Overview : Recombinant Human SPINT1 (Accession # NP_003701) Pro37-Glu449, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
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Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : Pro37
Form : Lyophilized from a 0.2 µm filtered solution in HEPES, NaCl and CaCl2.
Bio-activity : Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The IC50 is <2 nM, as measured under the described conditions.
Molecular Mass : Recombinant Human SPINT1, His-tagged has a calculated MW of 47 kDa. In SDS-PAGE migrates as 58 kDa, reducing conditions.
Protein length : Pro37-Glu449
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name SPINT1 serine peptidase inhibitor, Kunitz type 1 [ Homo sapiens ]
Official Symbol SPINT1
Synonyms SPINT1; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; kunitz-type protease inhibitor 1; HAI; MANSC2; HAI-1; hepatocyte growth factor activator inhibitor type 1; HAI1;
Gene ID 6692
mRNA Refseq NM_001032367
Protein Refseq NP_001027539
MIM 605123
UniProt ID O43278
Chromosome Location 15q13.3

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