Active Recombinant Human SPINT1 protein, His-tagged

• Cat.No.: SPINT1-1123H
• Product Overview: Recombinant Human SPINT1 protein(O43278-2)(Met1-Val433), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. IC50 value is < 2nM.
• Molecular Mass: The recombinant human SPINT1 consists of 409 amino acids and predictes a molecular mass of 45.8 kDa. In SDS-PAGE under reducing conditions, rh SPINT1 migrates as an approximately 55 kDa band due to glycosylation.
• Protein length: Met1-Val433
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SPINT1 serine peptidase inhibitor, Kunitz type 1 [ Homo sapiens ]
• Official Symbol: SPINT1
• Synonyms: SPINT1; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; kunitz-type protease inhibitor 1; HAI; MANSC2; HAI-1; hepatocyte growth factor activator inhibitor type 1; HAI1
• Gene ID: 6692
• mRNA Refseq: NM_001032367
• Protein Refseq: NP_001027539
• MIM: 605123
• UniProt ID: O43278