Active Recombinant Mouse Sema4d protein, His-tagged

• Cat.No.: Sema4d-4066M
• Product Overview: Recombinant Mouse Sema4d protein(NP_038688.2)(Met1-Arg733), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg733
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to bind human SEMA4A in a functional ELISA.
• Molecular Mass: The secreted recombinant mouse SEMA4D comprises 721 amino acids and has a calculated molecular mass of 80.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sema4d sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D [ Mus musculus ]
• Official Symbol: Sema4d
• Synonyms: SEMA4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; semaphorin-4D; sema J; M-sema G; semaphorin H; semaphorin-J; semaphorin-C-like 2; CD100; Semaj; Semcl2; coll-4; Semacl2
• Gene ID: 20354
• mRNA Refseq: NM_013660
• Protein Refseq: NP_038688