Active Recombinant Human SEMA4D protein, His-tagged

• Cat.No.: SEMA4D-7023H
• Product Overview: Recombinant Human SEMA4D protein(Met1-Arg734)(AAH54500.1), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg734
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to bind mouse CD45 hFc in functional ELISA.
• Molecular Mass: The recombinant human SEMA4D consists of 725 amino acids and predictes a molecular mass of 80.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSEMA4D is approximately 110 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SEMA4D sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D [ Homo sapiens ]
• Official Symbol: SEMA4D
• Synonyms: SEMA4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; C9orf164, chromosome 9 open reading frame 164 , SEMAJ; semaphorin-4D; CD100; coll 4; FLJ39737; M sema G; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; SEMAJ; coll-4; C9orf164; M-sema-G; FLJ33485; FLJ34282; FLJ46484; MGC169138; MGC169141
• Gene ID: 10507
• mRNA Refseq: NM_001142287
• Protein Refseq: NP_001135759
• MIM: 601866
• UniProt ID: Q92854