Active Recombinant Mouse Nrtn Protein (Cys101-Val195), N-His tagged, Animal-free, Carrier-free

• Cat.No.: Nrtn-72M
• Product Overview: Recombinant Mouse Nrtn Protein (Cys101-Val195) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Neurturin is a 22 kDa cytokine with 195 amino acid residues. Neurturin belongs to the same neurotrophic factor family as GDNF. Binding to GFRα-2 (GPI-linked receptor termed GDNF family receptor α-2), neurturin activates Ret receptor tyrosine kinase and MAPK pathway. It involves in neuron survival.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in SH-SY5Y cells. The ED50 for this effect is < 50 ng/mL.
• Molecular Mass: The protein has a calculated MW of 12.33 kDa.
  The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Cys101-Val195
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Nrtn neurturin [ Mus musculus (house mouse) ]
• Official Symbol: Nrtn
• Synonyms: NRTN; neurturin; NTN
• Gene ID: 18188
• mRNA Refseq: NM_008738
• Protein Refseq: NP_032764
• UniProt ID: P97463