Active Recombinant Mouse Notum Protein, His-tagged

• Cat.No.: NOTUM-25M
• Product Overview: Recombinant Mouse Notum (Ser88-Thr458, Cys337Ser)was expressed in mouse myeloma cell line with a C-terminal 6-His tag.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 88-458 a.a.
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.2-1.2 μg/mL in the presence of 0.1 μg/mL of Recombinant Mouse Wnt-3a.
• Molecular Mass: 39-46 kDa under reducing conditions.
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95% by SDS-PAGE
• Storage: 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.

Gene Information

• Gene Name: Notum notum pectinacetylesterase homolog (Drosophila) [ Mus musculus (house mouse) ]
• Official Symbol: NOTUM
• Synonyms: NOTUM; notum pectinacetylesterase homolog (Drosophila); protein notum homolog
• Gene ID: 77583
• mRNA Refseq: NM_175263.4
• Protein Refseq: NP_780472.3
• UniProt ID: Q8R116