Active Recombinant Mouse NBL1 protein(Met1-Asp178), His-tagged

• Cat.No.: NBL1-942M
• Product Overview: Recombinant Mouse NBL1 (Q61477) (Met 1-Asp 178) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Asp178
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells. The ED50 for this effect is 3-9 μg/mL.
• Molecular Mass: The secreted recombinant mouse NBL1 comprises 173 amino acids and has a calculated molecular mass of 18.8 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 28 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Nbl1 neuroblastoma, suppression of tumorigenicity 1 [ Mus musculus ]
• Official Symbol: NBL1
• Synonyms: NBL1; neuroblastoma, suppression of tumorigenicity 1; neuroblastoma suppressor of tumorigenicity 1; N03; zinc finger protein DAN; DAN; NO3; Dana; D4H1S1733E; MGC123430
• Gene ID: 17965
• mRNA Refseq: NM_008675
• Protein Refseq: NP_032701