Active Recombinant Human NBL1 protein(Met1-Asp180), His-tagged

• Cat.No.: NBL1-682H
• Product Overview: Recombinant Human DAN precursor (NP_005371.1) (Met 1-Asp 180) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Met1-Asp180
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 μg/ml in the presence of 25 ng/mL of recombinant human BMP4.
• Molecular Mass: The recombinant human DAN consists of 176 amino acids and has a calculated molecular mass of 19.3 kDa. As a result of glycosylation, rh DAN migrates as an approximately 27 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NBL1 neuroblastoma, suppression of tumorigenicity 1 [ Homo sapiens ]
• Official Symbol: NBL1
• Synonyms: NBL1; neuroblastoma, suppression of tumorigenicity 1; neuroblastoma suppressor of tumorigenicity 1; D1S1733E; DAN; DAND1; differential screening selected gene aberrant in neuroblastoma; NB; neuroblastoma candidate region; suppression of tumorigenicity 1; NO3; DAN domain family member 1; differential screening-selected gene aberrant in neuroblastoma; neuroblastoma candidate region, suppression of tumorigenicity 1; MGC8972
• Gene ID: 4681
• mRNA Refseq: NM_001204084
• Protein Refseq: NP_001191013
• MIM: 600613
• UniProt ID: P41271