Active Recombinant Mouse Laminin α4, His-tagged

• Cat.No.: LAMA4-6954M
• Product Overview: Recombinant Mouse Lamininα4 protein, was expressed in Mouse myeloma cell line, NS0derived Gln826-Ala1816,with a Cterminal 10-His tag

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 826-1816 a.a.
• Description: Laminin subunitalpha-4 is a protein that in humans is encoded by the LAMA4 gene. Laminins, afamily of extracellular matrix glycoproteins, are the major noncollagenousconstituent of basement membranes. They have been implicated in a widevariety of biological processes including cell adhesion, differentiation,migration, signaling, neurite outgrowth and metastasis. Laminins are composedof 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, andB2, respectively) and they form a cruciform structure consisting of 3 shortarms, each formed by a different chain, and a long arm composed of all 3chains. Each laminin chain is a multidomain protein encoded by a distinctgene. Several isoforms of each chain have been described
• MolecularMass: 112.0 kDa
• Purity: >90%, by SDS-PAGEunder reducing conditions and visualized by silver stain.
• Activity: Measured by theability of the immobilized protein to support the adhesion of HT1080 humanfibrosarcoma cells.The ED50 for this effect is typically 1-4μg/mL.
• EndotoxinLevel: <1.0 EU per 1 μgof the protein by the LAL method.
• N-terminalSequenceAnalysis: Mouse myeloma cellline, NS0 derived Gln826-Ala1816,with a Cterminal10 His tag
• Formulation: Lyophilized from a0.2 μm filtered solution in Tris and NaCl, pH 6.0.
• Reconstitution: Reconstitute at 200μg/mL in PBS.
• Storage: Use a manual defrostfreezer and avoid repeated freeze thaw Cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: Lama4 laminin, alpha 4 [ Musmusculus ]
• Official Symbol: Lama4
• Synonyms: Lama4; laminin,alpha 4; MGC141567; laminin subunit alpha-4; laminin [a]4; laminin-8 subunitalpha; laminin-9 subunit alpha; laminin-14 subunit alpha
• Gene ID: 16775
• mRNA Refseq: NM_010681
• Protein Refseq: NP_034811
• UniProt ID: P97927
• Chromosome Location: 10 B1; 10 25.0 cM
• Pathway: Africantrypanosomiasis; Amoebiasis; ECM-receptor interaction; Focal Adhesion; Focaladhesion; Pathways in cancer; Small cell lung cancer; Toxoplasmosis
• Function: receptor binding