Active Recombinant Mouse Interleukin 18

• Cat.No.: II18-272M
• Product Overview: Recombinant mouse interleukin 1, beta was expressed inE.coli,encoding the matured Mouse IL-18 protein sequence (corresponding to 36-192 aa).

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 36-192 a.a.
• Description: Interleukin 18 (IL-18) is an 18-kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24-kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-γ also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
• Purity: Greater than 90% purity as confirmed on SDS-PAGE by Coomassie brilliant blue staining.
• Activity: Induction of IFN-γ by mouse IL-18 receptor transfected KG-1 cell [human myelomonocyte; ATCC CCL246] in response to the recombinant mouse IL-18 was measured using human IFN-γ ELISA.
• Formulation: Lyophilized in PBS containing 1% sucrose. Reconstitute in 2 mL of ice-cold distilled water on ice.
• Storage: Lyophilized Mouse IL-18 is stable for 24 months from the date of manufacture when store at -20℃ or below. After reconstitution, avoid repeated freezing and thawing. The IL-18 can be stored for 1 week at 4℃. For storage, prepare appropriate aliquots and freeze them at -80℃.

Gene Information

• Gene Name: Il18 interleukin 18 [ Mus musculus ]
• Synonyms: Il18; interleukin 18; Igif; Il-18; Il18; interleukin 18; IL-1 gamma; interleukin-18; interleukin-1 gamma; IFN-gamma-inducing factor; interferon-gamma-inducing factor
• Gene ID: 16173
• mRNA Refseq: NM_008360
• Protein Refseq: NP_032386
• UniProt ID: P70380
• Chromosome Location: 9F;9 29.0 cM
• Pathway: Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway; NOD-like receptor signaling
• Function: cytokine activity

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.